Qualitative and quantitative HPLC/MS determination of proanthocyanidins in areca nut (Areca catechu)

被引：16
|
作者：
Wu, Qingli [1 ]
Yang, Yonghong [2 ]
Simon, James E. [1 ]
机构：
[1] Rutgers State Univ, Cook Coll, New Use Agr & Nat Plant Prod Program, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA
[2] Yunnan Agr Univ, Key Lab Inst Plant Pathol, Kunming 650201, Peoples R China
来源：
CHEMISTRY & BIODIVERSITY | 2007年 / 4卷 / 12期
关键词：
D O I：
10.1002/cbdv.200790231
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Proanthocyanidins (PACs) in areca nut (Areca catechu L.) were analyzed by high-performance liquid chromatography (HPLC) combined with electrospray-ionization mass spectrometry (ESI-MS) and compared to grape seed extract. Under optimized conditions, the separated PACs were individually analyzed and identified on the basis of their [M+H](+) peaks. The PAC distribution in areca nut was found to be very similar to that in grape seed, but lacking any gallate conjugates. Based on reverse-phase HPLC separation, the PAC monomers (+)-catechin (CA, 1) and (-)-epicatechin (EC; 2) were successfully quantified by ESI-MS in the selected-ion-monitoring (SIM) mode, (-)-epigallocatechin (EGC; 3) being used as internal standard. Detailed quality and validation assays showed that the accuracy and repeatability (n = 8) were within 10% for each analyte.
引用
收藏
页码：2817 / 2826
页数：10
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