Identification and characterization of a natural inter-genotypic (2b/1b) recombinant hepatitis C virus in Japan

被引：20
|
作者：
Yokoyama, Koji [1 ,2 ]
Takahashi, Masaharu [1 ]
Nishizawa, Tsutomu [1 ]
Nagashima, Shigeo [1 ]
Jirintai, Suljid [1 ]
Yotsumoto, Shigeru [1 ,2 ]
Okamoto, Hiroaki [1 ]
Momoi, Mariko Y. [2 ]
机构：
[1] Jichi Med Univ, Sch Med, Div Virol, Dept Infect & Immun, Shimotsuke, Tochigi 3290498, Japan
[2] Jichi Med Univ, Sch Med, Dept Pediat, Shimotsuke, Tochigi 3290498, Japan
来源：
ARCHIVES OF VIROLOGY | 2011年 / 156卷 / 09期
关键词：
MOLECULAR EPIDEMIOLOGY; GENETIC-RECOMBINATION; E2/NS1; PROTEIN; HCV GENOTYPES; ST-PETERSBURG; RNA VIRUSES; INFECTION; SEQUENCE; SUBTYPE; STRAINS;
D O I：
10.1007/s00705-011-1038-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A hepatitis C virus (HCV) strain (HC10-0804) recovered from a 12-year-old Japanese female with chronic hepatitis C segregated into discordant genotypes, 2b and 1b, in the 5'UTR/core and NS5B regions, respectively, thus suggesting an inter-genotypic recombination. The HC10-0804 isolate had a genomic length of 9,423 nucleotides (nt), excluding the poly(U) tract at the 3' terminus, and encoded a single open reading frame (ORF) for a polyprotein of 3,014 amino acids (aa). Based on Simplot and Bootscan analyses, the crossover point from 2b to 1b was estimated at nt 3443/3444 (aa 1034/1035), just after the beginning of the NS3 region. Comparison of the entire genomic sequence showed that the HC10-0804 strain was only 90.2% identical to the previously reported 2b/1b recombinant strain (SE-03-07-1689) from the Philippines, whose putative crossover point was 24 nt downstream of that of HC10-0804. These results indicate the circulation of a novel inter-genotypic (2b/1b) recombinant HCV in Japan.
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收藏
页码：1591 / 1601
页数：11
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