Arsenic Trioxide Induces Apoptosis in Uveal Melanoma Cells Through the Mitochondrial Pathway
被引:15
|
作者:
Chen, Miao-Ju
论文数: 0引用数: 0
h-index: 0
机构:
Show Chwan Mem Hosp, Dept Med Res, Changhua 500, TaiwanShow Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
Chen, Miao-Ju
[1
]
Yang, Pei-Yu
论文数: 0引用数: 0
h-index: 0
机构:
Show Chwan Mem Hosp, Dept Med Res, Changhua 500, TaiwanShow Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
Yang, Pei-Yu
[1
]
Ye, Yi-Zhen
论文数: 0引用数: 0
h-index: 0
机构:
Show Chwan Mem Hosp, Dept Med Res, Changhua 500, TaiwanShow Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
Ye, Yi-Zhen
[1
]
Hu, Dan-Ning
论文数: 0引用数: 0
h-index: 0
机构:
Show Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
New York Med Coll, New York Eye & Ear Infirm, New York, NY 10003 USAShow Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
Hu, Dan-Ning
[1
,2
]
Chen, Ming-Feng
论文数: 0引用数: 0
h-index: 0
机构:
Show Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
China Med Univ, Grad Inst Chinese Med Sci, Taichung 40402, TaiwanShow Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
Chen, Ming-Feng
[1
,3
]
机构:
[1] Show Chwan Mem Hosp, Dept Med Res, Changhua 500, Taiwan
[2] New York Med Coll, New York Eye & Ear Infirm, New York, NY 10003 USA
[3] China Med Univ, Grad Inst Chinese Med Sci, Taichung 40402, Taiwan
Uveal melanoma, the most common primary intraocular malignancy in adults, is highly resistant to most chemotherapeutic drugs. Arsenic trioxide (ATO) is known to inhibit ocular melanoma cell growth. However, the effects of ATO on human uveal melanoma cells are poorly understood. Therefore, this study evaluated the mechanisms of ATO and its inhibiting effects on a human uveal melanoma cell line (SP6.5). An MTT assay indicated that, compared to human fibroblasts, ATO had a stronger inhibiting effect on SP6.5 cell proliferation in a dose-and time-dependent manner. The apoptosis ratio in SP6.5 cells, which was indicated by cell DNA fragmentation, was 4.1-to 7.7-fold higher after ATO-treatment. The ATO treatment substantially increased the activities of caspase-3 and caspase-9, but not of caspase-8. These findings were consistent with the protein expression observed by Western blots. ATO also significantly enhanced expression of Bax and cytochrome c proteins but suppressed those of Bcl-2. Therefore, ATO-induced apoptosis in uveal melanoma cells occurs mainly through the mitochondrial pathway rather than through the death receptor pathway. This report is the first to evaluate the complete mitochondria-dependent apoptotic pathway of ATO in uveal melanoma cells. These results can be used to improve the clinical effectiveness of ATO treatment for uveal melanoma.
机构:
Chung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Sijhih Cathay Gen Hosp, Dept Ophthalmol, New Taipei, Taiwan
Cathay Gen Hosp, Dept Ophthalmol, Taipei, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Wang, Kai
Hu, Dan-Ning
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, New York Eye & Ear Infirm Mt Sinai, Tissue Culture Ctr, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, New York Eye & Ear Infirm Mt Sinai, Dept Ophthalmol, New York, NY 10029 USAChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Hu, Dan-Ning
Lin, Hui-Wen
论文数: 0引用数: 0
h-index: 0
机构:
Asia Univ, Dept Optometry, Taichung, Taiwan
China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Lin, Hui-Wen
Yang, Wei-En
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Chung Shan Med Univ Hosp, Dept Med Res, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Yang, Wei-En
Hsieh, Yi-Hsien
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Biochem Microbiol & Immunol, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Hsieh, Yi-Hsien
Chien, Hsiang-Wen
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Sijhih Cathay Gen Hosp, Dept Ophthalmol, New Taipei, Taiwan
Cathay Gen Hosp, Dept Ophthalmol, Taipei, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Chien, Hsiang-Wen
Yang, Shun-Fa
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
Chung Shan Med Univ Hosp, Dept Med Res, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110 Chien Kuo N Rd,Sect 1, Taichung, Taiwan
机构:
New York Eye & Ear Infirm, New York Med Coll, Tissue Culture Ctr, New York, NY 10003 USA
Jilin Univ, Hosp 1, Dept Ophthalmol, Changchun 130023, Peoples R ChinaNew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
Cui, Zhihua
Song, E.
论文数: 0引用数: 0
h-index: 0
机构:
Jilin Univ, Hosp 1, Dept Ophthalmol, Changchun 130023, Peoples R ChinaNew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
Song, E.
Hu, Dan-Ning
论文数: 0引用数: 0
h-index: 0
机构:
New York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
New York Eye & Ear Infirm, New York Med Coll, Tissue Culture Ctr, New York, NY 10003 USA
New York Eye & Ear Infirm, New York Med Coll, Dept Ophthalmol, New York, NY 10003 USANew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
Hu, Dan-Ning
Chen, Min
论文数: 0引用数: 0
h-index: 0
机构:
New York Eye & Ear Infirm, New York Med Coll, Tissue Culture Ctr, New York, NY 10003 USANew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
Chen, Min
Rosen, Richard
论文数: 0引用数: 0
h-index: 0
机构:
New York Eye & Ear Infirm, New York Med Coll, Dept Ophthalmol, New York, NY 10003 USANew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
Rosen, Richard
McCormick, Steven A.
论文数: 0引用数: 0
h-index: 0
机构:
New York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USANew York Eye & Ear Infirm, Dept Pathol, New York Med Coll, New York, NY 10003 USA
机构:
Jilin Univ, Hosp 1, Dept Ophthalmol, Changchun 130023, Peoples R China
New York Med Coll, New York Eye & Ear Infirm, Tissue Culture Ctr, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Lu, Chengwei
Song, E.
论文数: 0引用数: 0
h-index: 0
机构:
Jilin Univ, Hosp 1, Dept Ophthalmol, Changchun 130023, Peoples R ChinaNew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Song, E.
Hu, Dan-Ning
论文数: 0引用数: 0
h-index: 0
机构:
New York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
New York Med Coll, New York Eye & Ear Infirm, Tissue Culture Ctr, New York, NY 10003 USA
New York Med Coll, New York Eye & Ear Infirm, Dept Ophthalmol, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Hu, Dan-Ning
Chen, Min
论文数: 0引用数: 0
h-index: 0
机构:
New York Med Coll, New York Eye & Ear Infirm, Tissue Culture Ctr, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Chen, Min
Xue, Chunyan
论文数: 0引用数: 0
h-index: 0
机构:
New York Med Coll, New York Eye & Ear Infirm, Tissue Culture Ctr, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Xue, Chunyan
Rosen, Richard
论文数: 0引用数: 0
h-index: 0
机构:
New York Med Coll, New York Eye & Ear Infirm, Dept Ophthalmol, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
Rosen, Richard
McCormick, Steven A.
论文数: 0引用数: 0
h-index: 0
机构:
New York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USANew York Med Coll, New York Eye & Ear Infirm, Dept Pathol, New York, NY 10003 USA
机构:
Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China
Xuzhou Med Coll, Affiliated Hosp, Dept Stereotact Radiosurg, Xuzhou 221002, Peoples R ChinaXuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China
Jiang, Ai-Jun
Jiang, Guan
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China
Ctr Dis Control & Prevent Xuzhou City, Xuzhou 221006, Peoples R ChinaXuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China
Jiang, Guan
Li, Lian-Tao
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R ChinaXuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China
Li, Lian-Tao
Zheng, Jun-Nian
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R ChinaXuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Peoples R China