Effects of straw mulching on predatory myxobacterial communities in different soil aggregates under wheat-corn rotation

Crop straw mulching is an important organic supplement in sustainable agriculture; however, the effect of increased organic matter on the diversity of micropredators such as myxobacteria and the correlation between myxobacteria and microorganisms have been little explored. In the current investigation, high-throughput sequencing was performed to analyze the myxobacterial community composition in a wheat-corn rotation experimental field with 6-year straw mulching and fertilization treatments. The results reveal no significant influence of straw mulch application on myxobacterial alpha-diversity (P < 0.05). NMDS (nonmetric multidimensional scaling) and perMANOVA results indicate the significant influence of straw mulching application on myxobacterial community composition (P < 0.05), and several groups, including Haliangiaceae, Polyangiaceae, and Archangiaceae, also varied in soil aggregates. RDA (redundancy analysis) results show that TOC (total organic carbon) was the most important factor affecting the myxobacterial community structure. In addition, RDA and random forest analysis results show the contribution of myxobacterial community structure to soil bacterial community alpha- and beta-diversity, especially in the 0.25-1 mm and < 0.25 mm soil aggregate fractions. In conclusion, we suggest that the variation in myxobacterial community structure may be a driver of bacterial alpha- and beta-diversity in soil microhabitats and might be a cause of soil microbial community changes. Our results are fruitful for finding more efficient ways to use straw from waste for the betterment of sustainable agriculture by analyzing changes in myxobacterial community structure.