Contribution of microsatellites to sugar cane breeding program in mauritius

Sugar cane microsatellite libraries have recently been developed. Here, we report the efficiency of some microsatellite primers to generate polymorphic and easily scorable markers in sugar cane. Primers selected proved to be very efficient when applied to test for legitimacy of clones, to identify contaminants in nurseries and for diversity studies. We also investigated the amplification of microsatellite primers on DNA extracted using an alkaline treatment method, and this technique looks promising.