Characterization and Estimation of Genetic Diversity in Citrus Rootstocks

Thirty one genotypes representing 10 rootstocks of Citrus sp. were analyzed using 10 microsatellite and 17 Operon primers. The DNA analysis allowed the characterization of all samples and the detection of different level of polymorphisms. Specific markers, differentiating the rootstocks, were identified and could be used as marker assisted selection in breeding programs. The genetic diversity and relationships between the rootstocks revealed the highest value in sour orange, while the lowest one in Cleopatra mandarin. Data based on SSR and RAPD markers were used to estimate the genetic dissimilarity and to establish a dendrogram reflecting the genetic relationship between the different rootstocks. The dendrogram showed that all rootstocks were classified into tow main groups, the first formed of P. trifoliata and its hybrids, and the other consists of the rest of rootstocks. (C) 2011 Friends Science Publishers