Genetic engineering of Geobacillus spp.

被引：33

作者：

Kananaviciute, Ruta ^{[1
]}

Citavicius, Donaldas ^{[1
]}

机构：

[1] Vilnius Univ, Fac Nat Sci, Dept Microbiol & Biotechnol, LT-03101 Vilnius, Lithuania

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 111卷

关键词：

Geobacillus; Genetic transformation; Shuttle vector; Expression system; Genetic engineering; BACILLUS-STEAROTHERMOPHILUS NUB36; COMPLETE NUCLEOTIDE-SEQUENCE; ROLLING-CIRCLE PLASMIDS; GRAM-POSITIVE BACTERIA; KAUSTOPHILUS HTA426; SHUTTLE VECTOR; ESCHERICHIA-COLI; THERMOPHILIC BACILLI; GENUS GEOBACILLUS; PYRUVATE DECARBOXYLASE;

D O I：

10.1016/j.mimet.2015.02.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the genus Geobacillus are thermophiles that are of great biotechnological importance, since they are sources of many thermostable enzymes. Because of their metabolic versatility, geobacilli can be used as whole-cell catalysts in processes such as bioconversion and bioremediation. The effective employment of Geobacillus spp. requires the development of reliable methods for genetic engineering of these bacteria. Currently, genetic manipulation tools and protocols are under rapid development. However, there are several convenient cloning vectors, some of which replicate autonomously, while others are suitable for the genetic modification of chromosomal genes. Gene expression systems are also intensively studied. Combining these tools together with proper techniques for DNA transfer, some Geobacillus strains were shown to be valuable producers of recombinant proteins and industrially important biochemicals, such as ethanol or isobutanol. This review encompasses the progress made in the genetic engineering of Geobacillus spp. and surveys the vectors and transformation methods that are available for this genus. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：31 / 39

页数：9

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