Crystallization and preliminary X-ray diffraction analysis of pyranose 2-oxidase from the white-rot fungus Trametes multicolor

被引：8
|
作者：
Hallberg, BM
Leitner, C
Haltrich, D
Divne, C [1 ]
机构：
[1] Albanova Univ Ctr, KTH, Dept Biotechnol, SE-10691 Stockholm, Sweden
[2] Univ Nat Resources & Appl Life Sci, BOKU, Div Biochem Engn, Inst Food Technol, A-1190 Vienna, Austria
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷
关键词：
D O I：
10.1107/S0907444903024922
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyranose 2-oxidase (P2Ox) is a 270 kDa homotetrameric flavoenzyme that catalyzes the oxidation of D-glucose to 2-keto-D-glucose. P2Ox participates in lignin degradation by white-rot fungi and a tentative role of the enzyme is the production of H2O2 for lignin peroxidases. Crystals of Trametes multicolor P2Ox were grown from monomethylether PEG 2000, sodium acetate, MgCl2 and Ta6Br12. They belong to space group P2(1), with unit-cell parameters a = 99.9, b = 101.7, c = 135.6 Angstrom, beta = 90.85degrees. X-ray diffraction data to 2.0 Angstrom resolution were collected using synchrotron radiation. Self-rotation function calculations suggest that the asymmetric unit contains one homotetramer with 222 point-group symmetry.
引用
收藏
页码：197 / 199
页数：3
相关论文
共 50 条
  • [1] Pyranose oxidase from the white-rot fungus Trametes multicolor
    Haltrich, D
    Leitner, C
    Nidetzky, B
    Kulbe, KD
    Volc, J
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1999, 217 : U279 - U279
  • [2] Characterization of pyranose oxidase from the white-rot fungus Trametes multicolor
    Leitner, C
    Friessnegg, H
    Volc, J
    Nidetzky, B
    Kulbe, KD
    Haltrich, D
    7TH INTERNATIONAL CONFERENCE ON BIOTECHNOLOGY IN THE PULP AND PAPER INDUSTRY, VOL B: POSTER PRESENTATIONS, 1998, : B85 - B88
  • [3] Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor
    Leitner, C
    Volc, J
    Haltrich, D
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (08) : 3636 - 3644
  • [4] Crystal structure of pyranose 2-oxidase from the white-rot fungus Peniophora sp.
    Bannwarth, M
    Bastian, S
    Heckmann-Pohl, D
    Giffhorn, F
    Schulz, GE
    BIOCHEMISTRY, 2004, 43 (37) : 11683 - 11690
  • [5] Kinetic Mechanism of Pyranose 2-Oxidase from Trametes multicolor
    Prongjit, Methinee
    Sucharitakul, Jeerus
    Wongnate, Thanyaporn
    Haltrich, Dietmar
    Chaiyen, Pimchai
    BIOCHEMISTRY, 2009, 48 (19) : 4170 - 4180
  • [6] Reaction geometry and thermostable variant of pyranose 2-oxidase from the white-rot fungus Peniophora sp.
    Bannwarth, Michael
    Heckmann-Pohl, Dorothee
    Bastian, Sabine
    Giffhorn, Friedrich
    Schulz, Georg E.
    BIOCHEMISTRY, 2006, 45 (21) : 6587 - 6595
  • [7] Purification and characterization of a laccase from the white-rot fungus Trametes multicolor
    Leitner, C
    Hess, J
    Galhaup, C
    Ludwig, R
    Nidetzky, B
    Kulbe, KD
    Haltrich, D
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2002, 98 (1-9) : 497 - 507
  • [8] Purification and characterization of a laccase from the white-rot fungus Trametes multicolor
    Christian Leitner
    Johann Hess
    Christiane Galhaup
    Roland Ludwig
    Bernd Nidetzky
    Klaus D. Kulbe
    Dietmar Haltrich
    Applied Biochemistry and Biotechnology, 2002, 98-100 : 497 - 507
  • [9] Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics
    Chosrowjan, Haik
    Taniguchi, Seiji
    Wongnate, Thanyaporn
    Sucharitakul, Jeerus
    Chaiyen, Pimchai
    Tanaka, Fumio
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2012, 234 : 44 - 48
  • [10] Mutations of Thr169 affect substrate specificity of pyranose 2-oxidase from Trametes multicolor
    Spadiut, Oliver
    Leitner, Christian
    Tan, Tien-Chye
    Ludwig, Roland
    Divne, Christina
    Haltrich, Dietmar
    BIOCATALYSIS AND BIOTRANSFORMATION, 2008, 26 (1-2) : 120 - 127
12345