Immobilization of levan fructotransferase for the production of di-fructose anhydride from levan

被引：10
|
作者：
Lim, S
Lee, KY [1 ]
Lee, YB
Song, KB
机构：
[1] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Dept Mat & Biochem Engn, Kwangju 500757, South Korea
[3] Chonnam Natl Univ, Dept Pharm, Kwangju 500757, South Korea
[4] Chonnam Natl Univ, Fac Chem Engn, Kwangju 500757, South Korea
[5] Chonnam Natl Univ, Biotechnol Res Inst, Kwangju 500757, South Korea
[6] Korea Res Inst Biosci & Biotechnol, Microbial Metab Engn Res Unit, Taejon 305600, South Korea
[7] Korea Res Inst Biosci & Biotechnol, Genet Resources Ctr, Taejon 305600, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期
关键词：
adsorption; di-fructose anhydride; immobilization; ionic binding; levan fructotransferase;
D O I：
10.1023/A:1010549919579
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was immobilized on various carriers of which Chitopearl BCW2501 beads showed the higher activity of 320 U g(-1) for the formation of di-fructose anhydride compounds. The immobilized enzyme retained about 60% of its initial activity after being used for 20 cycles.
引用
收藏
页码：1335 / 1339
页数：5
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