Activation of DNA-hydrolyzing antibodies from the sera of autoimmune-prone MRL-lpr/lpr mice by different metal ions

We have shown previously that electrophoretically and immunologically homogeneous polyclonal IgGs from the sera of autoimmune-prone MRL mice possess DNase activity. Here we have analyzed for the first time activation of DNase antibodies (Abs) by different metal ions. Polyclonal DNase IgGs were not active in the presence of EDTA or after Abs dialysis against EDTA, but could be activated by several externally added metal (Me2+) ions, with the level of activity decreasing in the order Mn2+>= Mg2+>Ca2+>= Cu2+>Co2+>= Ni2+>Zn2+, whereas Fe2+ did not stimulate hydrolysis of supercoiled plasmid DNA (scDNA) by the Abs. The dependencies of the initial rate on the concentration of different Me2+ ions were generally bell-shaped, demonstrating one to four maxima at different concentrations of Me2+ ions in the 0.1-12 mM range, depending on the particular metal ion. In the presence of all Me2+ ions, IgGs pre-dialyzed against EDTA produced only the relaxed form of scDNA and then sequence-independent hydrolysis of relaxed DNA followed. Addition of Cu2+, Zn2+, or Ca2+ inhibited the Mg2+-dependent hydrolysis of scDNA, while Ni2+, Co2+, and Mn2+ activated this reaction. The Mn2+-dependent hydrolysis of scDNA was activated by Ca2+, Ni2+, Co2+, and Mg2+ ions but was inhibited by Cu2+ and Zn2+. After addition of the second metal ion, only in the case of Mg2+ and Ca2+ or Mn2+ ions an accumulation of linear DNA (single strand breaks closely spaced in the opposite strands of DNA) was observed. Affinity chromatography on DNA-cellulose separated DNase IgGs into many subtractions with various affinities to DNA and very different levels of the relative activity (0-100%) in the presence of Mn2+, Ca2+, and Mg2+ ions. In contrast to all human DNases having a single pH optimum, mouse DNase IgGs demonstrated several pronounced pH optima between 4.5 and 9.5 and these dependencies were different in the presence of Mn2+, Ca2+, and Mg2+ ions. These findings demonstrate a diversity of the ability of IgG to function at different pit and to be activated by different optimal metal cofactors. Possible reasons for the diversity of polyclonal mouse abzymes are discussed. (c) 2007 Elsevier B.V. All rights reserved.