Sesamin Ameliorates Insulin Resistance Induced by High Glucose/High Insulin in Hepatic L02 Cells

In this study, the antioxidant effect of sesamin on insulin resistance in hepatic L02 cells induced by high glucose/high insulin was investigated. Results showed that treatment with sesamin (200, 100 mu g/ml) increased the cell viability, activity of superoxide dismutase and glutathione peroxidase, and the content of reduced glutathione of L02 hepatocyte model of insulin-resistance. Moreover, treatment with sesamin decreased the content of malondialdehyde, production of nitric oxide, and the activity of nitric oxide synthase, inducible nitric oxide synthase, and gene expression levels of c-Jun NH2-terminal kinase significantly. The destructive change of hepatic L02 cells was ameliorated when treated with sesamin under microscopic observation. In conclusion, sesamin has anti-oxidative effect on the L02 hepatocyte model of insulin resistance induced by high glucose/high insulin.