Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

被引：15

作者：

Huska, Dalibor ^{[1
,2
]}

Adam, Vojtech ^{[1
]}

Babula, Petr ^{[3
]}

Trnkova, Libuse ^{[4
]}

Hubalek, Jaromir ^{[5
]}

Zehnalek, Josef ^{[1
]}

Havel, Ladislav ^{[2
]}

Kizek, Rene ^{[1
]}

机构：

[1] Mendel Univ, Dept Chem & Biochem, CZ-61300 Brno, Czech Republic

[2] Mendel Univ, Dept Plant Biol, CZ-61300 Brno, Czech Republic

[3] Univ Vet & Pharmaceut Sci, Fac Pharm, Dept Nat Drugs, CZ-61242 Brno, Czech Republic

[4] Masaryk Univ, Fac Sci, Dept Chem, CZ-61137 Brno, Czech Republic

[5] Brno Univ Technol, Fac Elect Engn & Commun, Dept Microelect, CZ-60200 Brno, Czech Republic

来源：

MICROCHIMICA ACTA | 2011年 / 173卷 / 1-2期

关键词：

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles; EARLY SOMATIC EMBRYOS; TRANSGENIC TOBACCO PLANTS; NORWAY SPRUCE; HEAVY-METALS; CADMIUM(II) IONS; DIPLOID PARTHENOGENESIS; VOLTAMMETRIC DETECTION; SALICYLIC-ACID; NUCLEIC-ACIDS; DNA;

D O I：

10.1007/s00604-011-0545-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

引用

页码：189 / 197

页数：9