Molecularly imprinted polymer solid phase extraction coupled with gas chromatography-mass spectrometry for the determination of trace clenbuterol hydrochloric in animal urine

The clenbuterol hydrochloric in animal urine was extracted and concentrated using molecularly imprinted polymer ( MIP) solid phase extraction cartridge and determined by gas chromatography -mass spectrometry ( selected ion mode, the selected ions are: 277, 262, 243 and 86) after derivatization of the purified sample with N, O-bis( trimethylsilyl) trifluoroacetamide ( BSTFA). The clean-up procedure was optimized, the clean effect and the matrix effect of the MIP cartridge were examined. A simple qualitative and quantitative analysis method has been developed for the determination of the Clenbuterol hydrochloric in animal urine sample. Under the optimized conditions, the limit of determination is 0.51 mu g/L and the limit of quantitative (LOQ) is 1. 00 mu g/L for urine sample, the recoveries of different quantities of clenbuterol hydrochloric are between 71. 0% and 89. 3 %, the relative standard deviations ( RSD) are 3. 2% - 9. 7%. there is a good linear correlation (the calibration coefficient is above 0. 9997) between the ratio of peak areas and the concentration of clenbuterol hydrochloric in the range of 10 - 1000 mu g/L. The developing method was applied to the determination of clenbuterol hydrochloric in real urine sample, the result is satisfactory comparing to the method of agricultural industrial standards, the precision of the method is high and the manipulation is simple and fast.