Self-Reporting Chemically Induced Protein Proximity System Based on a Malachite Green Derivative and the L5** Fluorogen Activating Protein

被引：5

作者：

Zeng, Guihua ^{[1
]}

Wang, Yi ^{[2
,3
]}

Bruchez, Marcel P. ^{[2
,3
]}

Liang, Fu-Sen ^{[1
]}

机构：

[1] Univ New Mexico, Dept Chem & Chem Biol, 300 Terrace St NE, Albuquerque, NM 87131 USA

[2] Carnegie Mellon Univ, Dept Biol Sci, Dept Chem, Pittsburgh, PA 15213 USA

[3] Carnegie Mellon Univ, Mol Biosensor & Imaging Ctr, Pittsburgh, PA 15213 USA

来源：

BIOCONJUGATE CHEMISTRY | 2018年 / 29卷 / 09期

基金：

美国国家卫生研究院;

关键词：

INDUCED DIMERIZATION; SPATIOTEMPORAL CONTROL; LIVING CELLS; IN-VIVO; SURFACE; ANTIBODIES; LIGANDS; LIBRARY; DOMAINS; PROBES;

D O I：

10.1021/acs.bioconjchem.8b00415

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A unique chemically induced proximity method is engineered based on mutant antibody VL domain using a fluorogenic malachite green derivative as the inducer, which gives fluorescent signals upon VL domain dimerization while simultaneously inducing downstream biological effects.

引用

页码：3010 / 3015

页数：6