High prevalence of opisthorchiasis in rural populations from Khammouane Province, central Lao PDR: serological screening using total IgG- and IgG4-based ELISA

被引：3

作者：

Phupiewkham, Weeraya ^{[1
,2
,3
,4
]}

Rodpai, Rutchanee ^{[1
,2
,3
]}

Inthavongsack, Somchith ^{[5
]}

Laymanivong, Sakhone ^{[6
]}

Thanchomnang, Tongjit ^{[7
]}

Sadaow, Lakkhana ^{[1
,2
,3
]}

Boonroumkaew, Patcharaporn ^{[1
,2
,3
]}

Sanpool, Oranuch ^{[1
,2
,3
]}

Janwan, Penchom ^{[8
]}

Intapan, Pewpan M. ^{[2
,3
]}

Maleewong, Wanchai ^{[1
,2
,3
]}

机构：

[1] Khon Kaen Univ, Fac Med, Dept Parasitol, Khon Kaen 40002, Thailand

[2] Khon Kaen Univ, Fac Med, Excellence Med Innovat Technol Res Grp, Khon Kaen 40002, Thailand

[3] Khon Kaen Univ, Mekong Hlth Sci Res Inst, Khon Kaen 40002, Thailand

[4] Rajamangala Univ Technol Tawan Ok, Fac Sci & Technol, Dept Sci & Math, Chon Buri 20110, Thailand

[5] Thakhek & Hlth Khammouane Prov, Malaria & Helminth Control Ctr, Thakhek 12000, Khammouane Prov, Laos

[6] Minist Hlth, Ctr Malariol Parasitol & Entomol, Vientiane Capital 01000, Laos

[7] Mahasarakham Univ, Fac Med, Maha Sarakham 44000, Thailand

[8] Walailak Univ, Sch Allied Hlth Sci, Dept Med Technol, Nakhon Si Thammarat 80161, Thailand

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2021年 / 115卷 / 12期

关键词：

eggs per gram; ELISA; IgG; IgG4; Opisthorchis viverrini; seroprevalence; ANTIBODY-RESPONSE; VIVERRINI; SERUM; EPIDEMIOLOGY; INFECTION; PARASITE; CHOLANGIOCARCINOMA; ANTIGEN; URINE; AREA;

D O I：

10.1093/trstmh/trab066

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Background: Human opisthorchiasis, caused by Opisthorchis viverrini, is a public health problem in Southeast Asia and a major risk factor for cholangiocarcinoma. In Lao PDR, seroprevalence and the relationship between the number of O. viverrini eggs in infected people and specific antibody responses are still unknown. We evaluated and compared parasitological and serological screening methods in the community in an endemic area of opisthorchiasis in Lao PDR. Methods: Seroprevalence of O. viverrini-specific total IgG and IgG4 antibodies and their relationships with O. viverrini egg intensities were evaluated in Khammouane Province, central Lao PDR, using ELISA and a modified formalin ethyl-acetate concentration technique (FECT). Results: FECT stool examination revealed O. viverrini eggs in 70.3% (90/128) of individuals (95% CI 61.6 to 78.1%) while ELISA (based on total IgG and on IgG4 antibodies to O. viverrini) found 98.4% (95% CI 94.5 to 99.8%) and 89.8% (95% CI 83.3 to 94.5%) of sera, respectively. There was a positive and significant correlation between numbers of O. viverrini eggs per gram and levels of both IgG (R-2=0.168, p<0.001) and IgG4 (R-2=0.219, p<0.001) antibodies. Conclusions: A high prevalence of human opisthorchiasis in Lao PDR was found using a new platform, serological screening in the community. This points to a need for sustainable control of this liver fluke infection.

引用

页码：1403 / 1409

页数：7