High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells

被引：268
|
作者：
Raymond, Christopher S. [1 ]
Soriano, Philippe [1 ]
机构：
[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Program Dev Biol, Seattle, WA 98104 USA
来源：
PLOS ONE | 2007年 / 2卷 / 01期
关键词：
D O I：
10.1371/journal.pone.0000162
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and Phi C31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and Phi C31 (Phi C31o) SSRs, which result in recombination efficiencies similar to Cre.
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页数：4
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