Production, purification and characterization of two recombinant DNA-derived N-terminal ovine growth hormone variants: oGH3 and oGH5

被引：0
|
作者：
Sami, Amtul Jamil [1 ]
Wallis, O. C. [2 ]
Wallis, M. [2 ]
机构：
[1] Univ Punjab, Inst Biochem & Biotechnol, Lahore, Pakistan
[2] Univ Sussex, Sch Life Sci, Dept Biochem, Brighton BN1 9QG, E Sussex, England
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2008年 / 7卷 / 12期
关键词：
growth hormone; GH; oGH3; oGH5; radioimmuno assay; radio receptor assay;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two recombinant DNA-derived variants of ovine growth hormone were produced, purified, characterized and compared with the authentic pituitary derived GH. The variants oGH3 and oGH5 were isolated by differential centrifugation method and were purified after refolding by ion-exchange chromatography and gel filtration. Both the proteins showed single band on SDS-PAGE and had molecular weight and iso-electric point closer to authentic pituitary GH. The variants oGH3 and oGH5 were compared with the authentic pituitary derived GH in radio immuno assays, radio receptor assays and binding with the monoclonal antibodies OA11 and OA12.
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页码：1859 / 1864
页数：6
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