Purpose: To evaluate the effect of the inclusion of glutaraldehyde on the shear bond strength of new ethanol-based adhesives (Gluma Comfort Bond & Desensitizer, GCBD and Gluma Comfort Bond, GCB) versus acetone-based Gluma One Bond (GOB) and a conventional three-step adhesive as a control. Materials and Methods: Forty bovine incisors were mounted in acrylic, polished to 600-grit, and randomly assigned to four groups (n=10). Dentin was etched for 15 s using 35% phosphoric acid, rinsed, and lightly dried. After adhesive application and curing, Charisma composite was applied in a #5 gelatin capsule and light-cured. Specimens were loaded in shear using an Instron at 5 mm/min. Shear bond strengths were calculated by dividing failure load by bonded surface area. ANOVA showed a significant difference in means at P < 0.01, so Tukey's test was used for pairwise comparisons. Results: Mean SBS (+/- SD, MPa) were: GOB: 8 (4.4)(ab); GCB: TO (4.4)(ab); GCBD: 14 (6)(bc); SBMP: 17.7 (6.4)(c). Superscript letters indicate Tukey's homogeneous subsets (P < 0.05).