Aberrant expression of LncRNA-MIR31HG regulates cell migration and proliferation by affecting miR-31 and miR-31*in Hirschsprung's disease

被引:33
|
作者
Cai, Peng [1 ]
Li, Hongxing [2 ,3 ]
Huo, Weiwei [4 ]
Zhu, Hairong [5 ]
Xu, Chao [6 ]
Zang, Rujin [2 ,3 ]
Lv, Wei [7 ]
Xia, Yankai [1 ,3 ]
Tang, Weibing [2 ,3 ]
机构
[1] Soochow Univ, Childrens Hosp, Suzhou, Peoples R China
[2] Nanjing Med Univ, Dept Pediat Surg, Childrens Hosp, Nanjing 210008, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Sch Publ Hlth, Minist Educ, Key Lab Modern Toxicol, Nanjing, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Affiliated Suzhou Hosp, Suzhou Municipal Hosp, Dept Gynecol, Suzhou, Peoples R China
[5] Fudan Univ, Dept Gastroenterol, Zhongshan Hosp, Shanghai, Peoples R China
[6] Nanjing Med Univ, Huaian Peoples Hosp 1, Huaian, Peoples R China
[7] Nanjing Univ, Sch Business, Nanjing, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
gene regulation; Hirschsprung's disease; lncRNA; miRNAs; neural crest cell; pathway; LONG NONCODING RNAS; NERVOUS-SYSTEM DEVELOPMENT; GENE; MICRORNA-31;
D O I
10.1002/jcb.26830
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hirschsprung's disease (HSCR) is a birth defect that causes a failure of the enteric nervous system to cover the distal gut during early embryonic development. Evidence shows that long non-coding RNAs (lncRNA) play important roles in HSCR. The MIR31 host gene (MIR31HG), also known as Loc554202, is a long non-coding RNA (lncRNA), which acts as the host gene of (microRNA) miR-31 and miR-31*. There have been no studies regarding its function in early developmental defects during pregnancy, and its downstream genetic receptors. We report that downregulation of MIR31HG inhibited migration and proliferation in 293T and SH-SY5Y cell lines, by suppressing miR-31 and miR-31*. Moreover, the downregulation of miR-31 and miR-31* enhanced inter-alpha-trypsin inhibitor heavy chain 5 (ITIH5) and the phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic gamma subunit (PIK3CG), respectively with reductions of cell migration and proliferation in 293T and SH-SY5Y cell lines. In addition, synergistic actions were observed between miR-31 and miR-31* in cell migration and proliferation. Our results demonstrated that the MIR31HG-miR-31/31*-ITIH5/PIK3CG pathway plays a role in the pathogenesis of HSCR.
引用
收藏
页码:8195 / 8203
页数:9
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