Comprehensive Analysis of Differentially Expressed mRNAs, lncRNAs and circRNAs Related to Intramuscular Fat Deposition in Laiwu Pigs

被引:2
|
作者
Li, Jingxuan [1 ]
Zhao, Xueyan [1 ]
Wang, Yanping [1 ]
Wang, Jiying [1 ]
机构
[1] Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Prov Key Lab Anim Dis Control & Breeding, Jinan 250108, Peoples R China
关键词
message RNA (mRNAs); long noncoding RNAs (lncRNAs); circular RNAs (circRNAs); intramuscular fat (IMF); pigs; QUANTITATIVE TRAIT LOCI; SIGNALING PATHWAY; ACID-COMPOSITION; NONCODING RNA; CIRCULAR RNAS; MEAT QUALITY; PPAR-GAMMA; ADIPOGENESIS; IDENTIFICATION; TRANSCRIPTION;
D O I
10.3390/genes13081349
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) are important classes of small noncoding RNAs that can regulate numerous biological processes. To understand the role of message RNA (mRNAs, lncRNAs and circRNAs) in the regulation of intramuscular fat (IMF) deposition, in this study the expression profiles of longissimus dorsi (LD) muscle from six Laiwu pigs (three with extremely high and three with extremely low IMF content) were sequenced based on rRNA-depleted library construction. In total, 323 differentially expressed protein-coding genes (DEGs), 180 lncRNAs (DELs) and 105 circRNAs (DECs) were detected between the high IMF and low IMF groups. Functional analysis indicated that most DEGs, and some target genes of DELs, were enriched into GO terms and pathways related to adipogenesis, suggesting their important roles in regulating IMF deposition. In addition, 12 DELs were observed to exhibit a positive relationship with stearoyl-CoA desaturase (SCD), phosphoenolpyruvate carboxykinase 1 (PCK1), and adiponectin (ADIPOQ), suggesting they are highly likely to be the target genes of DELs. Finally, we constructed a source gene-circRNA-miRNA connective network, and some of miRNA of the network have been reported to affect lipid metabolism or adipogenesis. Overall, this work provides a valuable resource for further research and helps to understand the potential functions of lncRNAs and circRNAs in IMF deposition.
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页数:14
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