Analysis of Rab GTPase and GTPase-activating protein function at primary cilia

被引：6
|
作者：
Yoshimura, Shin-ichiro [1 ]
Haas, Alexander K. [1 ]
Barr, Francis A. [1 ]
机构：
[1] Univ Liverpool, Canc Res Ctr, Liverpool L69 3BX, Merseyside, England
来源：
SMALL GTPASES IN DISEASE, PT B | 2008年 / 439卷
关键词：
D O I：
10.1016/S0076-6879(07)00426-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Primary cilia are sensory structures on the cell surface whose formation requires tight integration of microtubutes and polarized membrane trafficking events. Rabs are GTP-binding proteins of the Ras superfamily that control directed membrane trafficking by regulating membrane-membrane and membrane-cytoskeleton interactions. This chapter describes cell biological and biochemical methods for the analysis of Rab function and Rab GTPase-activating proteins during primary cilia formation.
引用
收藏
页码：353 / 364
页数：12
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