Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.)

被引：21
|
作者：
Srinivas, L. [1 ]
Kumar, G. B. Sunil [1 ]
Ganapathi, T. R. [1 ]
Revathi, C. J. [2 ]
Bapat, V. A. [1 ]
机构：
[1] Bhabha Atom Res Ctr, Plant Cell Culture Technol Sect, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India
[2] Shantha Biotechn Ltd, Hyderabad 501401, Andhra Pradesh, India
来源：
PLANT BIOTECHNOLOGY REPORTS | 2008年 / 2卷 / 01期
关键词：
agroinfiltration; edible vaccine; ER; retention signal; hepatitis B surface antigen; tomato;
D O I：
10.1007/s11816-008-0041-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus 's' gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D. W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.
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页码：1 / 6
页数：6
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