Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin

被引:2
|
作者
Chen, Jin [1 ]
Gong, Min [1 ]
Huang, Zhuo [1 ]
Wang, Fang [2 ]
Wang, Yajing [3 ]
Hu, Zuquan [1 ]
Zeng, Zhu [1 ]
Wang, Yun [2 ]
机构
[1] Guizhou Med Univ, Key Lab Biol & Med Engn, Sch Biol & Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang 550025, Peoples R China
[2] Guizhou Med Univ, Sch Basic Med Sci, Key Lab Infect Immune & Antibody Engn Guizhou Pro, Guiyang 550025, Peoples R China
[3] Guizhou Med Univ, Affiliated Stomatol Hosp, Guiyang 550025, Peoples R China
来源
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
beta-lactoglobulin; aspirin; gastric injury; static quenching; molecular docking; BOVINE SERUM-ALBUMIN; DRUG; SPECTROSCOPY; ANTICANCER; COMPLEX; ULCER; ACID; PH;
D O I
10.2147/DDDT.S351100
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Y Purpose: Gastric injury is a major issue for long-term administration of aspirin. In this work, we tried to explore the possibility of using BLG to alleviate aspirin-induced gastric injury, because of excellent abilities of BLG in loading drug molecules. Methods: Various spectroscopic techniques and molecular docking methods were applied to investigate the interaction mechanism between BLG and aspirin. Animal experiments were performed to figure out the effects of taking aspirin-BLG on the stomach. Results: Our results demonstrate that aspirin could bind with BLG to form stable aspirin-BLG complex (the binding constant K-b = 2.051 x 10(3) M-1). The formation process is endothermic (Delta H>0) and the main acting force is hydrophobic force. Our data also show that the aspirin-BLG complex is formed with a higher affinity in simulated gastric fluid and could remain stable for several hours, which might arise from its special binding mode under acidic condition and the resistance of BLG to gastric digestion. Furthermore, animal models (rats with aspirin-induced gastric damage) were built. The results of animal experiments reveal that the oral administration of aspirin-BLG could cause less damage to gastric tissue, and it also hardly triggers obvious inflammatory responses. Conclusion: This study would contribute to an in-depth understanding of the interaction mechanism between BLG and aspirin. It is reasonable to believe that using BLG to bind with aspirin would be a potential way to alleviate the aspirin-induced gastric injury.
引用
收藏
页码:571 / 586
页数:16
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