A fluorescent ELISA based on the enzyme-triggered synthesis of poly (thymine)-templated copper nanoparticles

被引：39

作者：

Sun, Jian ^{[1
]}

Hu, Tao ^{[1
,2
]}

Xu, Xiaolong ^{[1
]}

Wang, Lei ^{[1
]}

Yang, Xiurong ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China

来源：

NANOSCALE | 2016年 / 8卷 / 38期

基金：

中国国家自然科学基金;

关键词：

IMMUNOSORBENT-ASSAY ELISA; ALKALINE-PHOSPHATASE; ULTRASENSITIVE DETECTION; PLASMONIC ELISA; QUANTUM DOTS; IMMUNOASSAY; NANOMATERIALS; BIOMARKERS; STRATEGY; PROBES;

D O I：

10.1039/c6nr06446a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Inspired by the ALP-triggered hydrolysis of the substrate ascorbic acid 2-phosphate as well as the product ascorbate-controlled generation of polyT-CuNPs with bright red fluorescence, we have developed a novel, selective and sensitive fluorescence turn-on assay for ALP activity sensing. Based on the conventional ELISA platform and commercially available antibody-ALP conjugates, an unconventional fluorescent ELISA system has been rationally developed and successfully applied in the quantitative measurement of model antigen proteins using fluorescence spectroscopy and a naked-eye readout under ultraviolet light.

引用

页码：16846 / 16850

页数：5

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