Effect of temperature on gene expression of the GH/IGF system during embryonic development in rainbow trout (Oncorhynchus mykiss)

被引:37
|
作者
Gabillard, JC [1 ]
Rescan, PY [1 ]
Fauconneau, B [1 ]
Weil, C [1 ]
Le Bail, PY [1 ]
机构
[1] INRA, SCRIBE, Stn Commune Rech Ichtyophysiol Biodiversite & Env, Equipe Croissance & Qual Chair Poissons, F-35042 Rennes, France
关键词
D O I
10.1002/jez.a.10280
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
In fish, the GH/IGF system installs very early during development suggesting that this system could promote embryonic growth and development. In contrast to mammals, the embryonic growth rate of poikilotherms depends considerably on the incubation temperature. Therefore, the aim of this study was to determine if variations of embryo growth in response to temperature could be associated with modifications in the gene expression of the GH/IGF system. In this study, using whole mount in situ hybridisation, we demonstrated that embryo incubation temperature (4, 8, and 12degreesC) did not change the timing of GH-1 and GH-2 mRNA expression in somatotroph cells (stage 24). Similarly, at hatching (stage 30), we did not observe an obvious difference in GH protein and GH-1 and GH-2 transcript amounts in relation to the incubation temperature. Furthermore, from stage 22 to 25, the highest temperature led to a specific up-regulation of IGF-2 (2-fold between 4 and 12degreesC), and both IGF-RIa and IGFRIb mRNA (1.5-fold between 4 and 12degreesC), while no difference was observed for IGF-1 mRNA. Conversely, at hatching, the highest temperature specifically down-regulated IGF-2 (3fold between 4 and 12degreesC) and both IGF receptor mRNAs (2 fold between 4 and 12degreesC) present in the head, while no difference was observed in the trunk. Our results demonstrated that different incubation temperatures during trout embryonic development did not change the stage of somatotroph cell appearance. Before hatching, IGF-2 and both IGF receptors, but not IGF-1 mRNA, were specifically up-regulated by high temperatures and could be related to the enhancement of embryonic growth rate. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:134 / 142
页数:9
相关论文
共 50 条
  • [1] Differential expression of the two GH genes during embryonic development of rainbow trout Oncorhynchus mykiss in relation with the IGFs system
    Gabillard, JC
    Duval, H
    Cauty, C
    Rescan, PY
    Weil, C
    Le Bail, PY
    [J]. MOLECULAR REPRODUCTION AND DEVELOPMENT, 2003, 64 (01) : 32 - 40
  • [2] Coordinated regulation of the GH/IGF system genes during refeeding in rainbow trout (Oncorhynchus mykiss)
    Gabillard, Jean-Charles
    Kamangar, Barzan Bahrami
    Montserrat, Nuria
    [J]. JOURNAL OF ENDOCRINOLOGY, 2006, 191 (01) : 15 - 24
  • [3] Effect of water temperature on the embryonic development and hatching results of rainbow trout (Oncorhynchus mykiss)
    Lefler Kinga
    Gal Janos
    Demeter Gyongyi
    [J]. MAGYAR ALLATORVOSOK LAPJA, 2006, 128 (09) : 565 - 569
  • [4] Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss):: Their expression during early embryonic development
    Ramachandra, Raghuveer K.
    Salem, Mohamed
    Gahr, Scott
    Rexroad, Caird E., III
    Yao, Jianbo
    [J]. BMC DEVELOPMENTAL BIOLOGY, 2008, 8
  • [5] Embryonic expression of UCP2 in rainbow trout (Oncorhynchus mykiss)
    I. Coulibaly
    S. A. Gahr
    J. Yao
    C. E. Rexroad
    [J]. Fish Physiology and Biochemistry, 2006, 32 : 249 - 253
  • [6] Gene expression in the liver of rainbow trout, Oncorhynchus mykiss, during the stress response
    Momoda, T. S.
    Schwindt, A. R.
    Feist, G. W.
    Gerwick, L.
    Bayne, C. J.
    Schreck, C. B.
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2007, 2 (04): : 303 - 315
  • [7] Transcription factor profiling during early embryonic development in rainbow trout (Oncorhynchus mykiss).
    Ramachandra, Raghuveer
    Yao, Jianbo
    [J]. BIOLOGY OF REPRODUCTION, 2006, : 127 - 127
  • [8] Embryonic expression of UCP2 in rainbow trout (Oncorhynchus mykiss)
    Coulibaly, I.
    Gahr, S. A.
    Yao, J.
    Rexroad, C. E., III
    [J]. FISH PHYSIOLOGY AND BIOCHEMISTRY, 2006, 32 (03) : 249 - 253
  • [9] Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss)
    Mohamed Salem
    Jeff Silverstein
    Caird E Rexroad
    Jianbo Yao
    [J]. BMC Genomics, 8
  • [10] Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss)
    Salem, Mohamed
    Silverstein, Jeff
    Rexroad, Caird E., III
    Yao, Jianbo
    [J]. BMC GENOMICS, 2007, 8 (1)