Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker

被引：19

作者：

Mo, Xuejun ^{[1
,2
]}

Li, Qiushi ^{[2
,3
]}

Lui, Lena Wai Yi ^{[4
]}

Zheng, Baixue ^{[2
,5
]}

Kang, Chiang Huen ^{[2
]}

Nugraha, Bramasta ^{[2
,6
]}

Yue, Zhilian ^{[2
]}

Jia, Rui Rui ^{[7
]}

Fu, Hong Xia ^{[8
]}

Choudhury, Deepak ^{[2
,6
]}

Arooz, Talha ^{[2
]}

Yan, Jie ^{[8
]}

Lim, Chwee Teck ^{[3
,4
,5
,6
,9
,10
,12
]}

Shen, Shali ^{[7
]}

Tan, Choon Hong ^{[1
,6
]}

Yu, Hanry ^{[2
,3
,5
,6
,7
,11
,12
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Fac Sci, Singapore 117576, Singapore

[2] ASTAR, Inst Bioengn & Nanotechnol, Singapore 138669, Singapore

[3] Natl Univ Singapore, Temasek Labs, Res Ctr Excellence Mechanobiol, Singapore 117411, Singapore

[4] Singapore MIT Alliance Res & Technol, ID IRG, Singapore 117456, Singapore

[5] Singapore MIT Alliance, Computat & Syst Biol Program, Singapore 117576, Singapore

[6] NUS Grad Sch Integrat Sci, Ctr Life Sci CeLS, Singapore 117456, Singapore

[7] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117597, Singapore

[8] Natl Univ Singapore, Fac Sci, Dept Phys, Singapore 117576, Singapore

[9] Natl Univ Singapore, Fac Engn, Div Bioengn, Singapore 117576, Singapore

[10] Natl Univ Singapore, Fac Engn, Dept Mech Engn, Singapore 117576, Singapore

[11] Natl Univ Singapore, DSO Labs, NUS Tissue Engn Programme, Singapore 117597, Singapore

[12] Singapore MIT Alliance Res & Technol, Singapore 117543, Singapore

来源：

BIOMATERIALS | 2010年 / 31卷 / 29期

关键词：

Rapid dendrimer; Oleyl poly (ethylene glycol) (oleyl-PEG); Cell- ECM interaction; Multicellular structures; Defined cellular structures; Tissue engineering; MULTICELLULAR SPHEROIDS; TISSUE; BIOMATERIALS; ARCHITECTURE; AGGREGATION; HEPATOCYTES; ADHESION; DESIGN; CELLS;

D O I：

10.1016/j.biomaterials.2010.06.020

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Tissue constructs that mimic the in vivo cell-cell and cell-matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We demonstrate rapid assembly of C3A cells into multi- cell structures using a dendrimeric intercellular linker. The linker is composed of oleyl- polyethylene glycol (PEG) derivatives conjugated to a 16 arms- polypropylenimine hexadecaamine (DAB) dendrimer. The positively charged multivalent dendrimer concentrates the linker onto the negatively charged cell surface to facilitate efficient insertion of the hydrophobic oleyl groups into the cellular membrane. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs. We constructed defined multi- cellular structures such as rings, sheets or branching rods that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for biomedical applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：7455 / 7467

页数：13