Technical factors of artemisin biosynthesis in Artemisia annua hairy root culture

The optimum cultural conditions of growth and artemisinin accumulation of hairy roots in Artemisia annua L. were identified as follows: the initial pH 5.8 similar to 6.0; the rate of flask shaking, 130 similar to 150 r/min; the liquid medium volume per flask, 25%; light cycle, 16 h/d; temperature, 30 degrees C. Under the above conditions, up to 233.3 mg/L of artemisinin could be obtained after 25 days of culture.