Determination of robenidine in eggs by liquid chromatography with UV spectrophotometric detection

A method has been developed to analyse for robenidine drug residues in eggs. Egg samples were extracted with acetonitrile and the sample extracts were defatted with hexane and aliquots of the extracts were analysed by liquid chromatography with UV spectrophotometric detection (317nm). The method was validated according to the criteria defined in Commission Decision 2002/657/EC. The validation parameters linearity, precision, recovery, specificity, decision limit (CC alpha) and detection capability (CC beta) were determined. The decision limit was 10 mu g kg(-1) and the detection capability was 17 mu g kg(-1). The results of the inter-assay study, which was performed by fortifying egg samples (n = 6) at three levels in three separate assays, show mean recoveries to be between 79 and 105%. The precision of the method, expressed as CV (%) values for the within-laboratory repeatability, was <= 13.5%. (c) 2005 Elsevier B.V. All rights reserved.