Metabolic engineering for the synthesis of steviol glycosides: current status and future prospects

被引：19

作者：

Zhou, Xuan ^{[1
,2
]}

Gong, Mengyue ^{[3
]}

Lv, Xueqin ^{[1
,2
]}

Liu, Yanfeng ^{[1
,2
]}

Li, Jianghua ^{[1
,2
]}

Du, Guocheng ^{[1
,2
]}

Liu, Long ^{[1
,2
]}

机构：

[1] Jiangnan Univ, Sci Ctr Future Foods, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China

[3] Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2021年 / 105卷 / 13期

基金：

中国国家自然科学基金;

关键词：

Steviol glycosides; Biosynthetic pathways; Metabolic engineering; Enzyme Engineering; Uridine diphosphate glycosyltransferases; METHYLERYTHRITOL PHOSPHATE-PATHWAY; ENT-KAURENOIC ACID; HETEROLOGOUS EXPRESSION; ISOPRENOID BIOSYNTHESIS; REBAUDIANA BERTONI; REBAUDIOSIDE D; 76G1; GENE; SWEETENER; GLYCOSYLTRANSFERASES; GIBBERELLIN;

D O I：

10.1007/s00253-021-11419-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the pursuit of natural non-calorie sweeteners, steviol glycosides (SGs) have become one of the most popular natural sweeteners in the market. The SGs in Stevia are a mixture of SGs synthesized from steviol (a terpenoid). SGs are diterpenoids. Different SGs depend on the number and position of sugar groups on the core steviol backbone. This diversity comes from the processing of glycoside steviol by various glycosyltransferases. Due to the differences in glycosylation, each SG has unique sensory properties. At present, it is more complicated to extract high-quality SGs from plants, so the excavation of the metabolic pathways of engineered microorganisms to synthesize SGs has been extensively studied. Specifically, the expression of different glycosyltransferases in microbes is key to the synthesis of various SGs by engineered microorganisms. To trigger more researches on the functional characterization of the enzymes encoded by these genes, this review describes the latest research progresses of the related enzymes involved in SG biosynthesis and metabolic engineering.

引用

页码：5367 / 5381

页数：15

共 50 条

[1] Metabolic engineering for the synthesis of steviol glycosides: current status and future prospects
Xuan Zhou
Mengyue Gong
Xueqin Lv
Yanfeng Liu
Jianghua Li
Guocheng Du
Long Liu
[J]. Applied Microbiology and Biotechnology, 2021, 105 : 5367 - 5381
[2] Genetic engineering of millets: current status and future prospects
S. Antony Ceasar
S. Ignacimuthu
[J]. Biotechnology Letters, 2009, 31 : 779 - 788
[3] Genetic engineering of millets: current status and future prospects
Ceasar, S. Antony
Ignacimuthu, S.
[J]. BIOTECHNOLOGY LETTERS, 2009, 31 (06) : 779 - 788
[4] Plastome engineering in vegetable crops: current status and future prospects
Yarra, Rajesh
[J]. MOLECULAR BIOLOGY REPORTS, 2020, 47 (10) : 8061 - 8074
[5] Plastome engineering in vegetable crops: current status and future prospects
Rajesh Yarra
[J]. Molecular Biology Reports, 2020, 47 : 8061 - 8074
[6] Genome engineering in ornamental plants: Current status and future prospects
Kishi-Kaboshi, Mitsuko
Aida, Ryutaro
Sasaki, Katsutomo
[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2018, 131 : 47 - 52
[7] Current status and future prospects
Marks, J
[J]. GUT FLORA AND HEALTH - PAST, PRESENT AND FUTURE, 1996, (219): : 57 - 64
[8] Current status and future prospects
Brettle, Alison
[J]. HEALTH INFORMATION AND LIBRARIES JOURNAL, 2008, 25 : 32 - 34
[9] Current status and future prospects
McKibbon, K. Ann
Gamsby, Meghan K.
[J]. HEALTH INFORMATION AND LIBRARIES JOURNAL, 2008, 25 : 66 - 67
[10] Current status and future prospects of protein engineering especially of computational analysis
Yao, T
[J]. PROTEIN ENGINEERING, 1995, 8 (09): : 38 - 38

← 1 2 3 4 5 →