Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells

被引：33
|
作者：
Wu, Yanan [1 ]
Meng, Hong-Min [1 ]
Chen, Juan [1 ]
Jiang, Kemei [1 ]
Yang, Ran [1 ]
Li, Yingying [1 ]
Zhang, Ke [2 ]
Qu, Lingbo [1 ]
Zhang, Xiao-Bing [3 ]
Li, Zhaohui [1 ]
机构：
[1] Zhengzhou Univ, Inst Chem Biol & Clin Applicat, Affiliated Hosp 1,Coll Chem, Henan Joint Int Res Lab Green Construct Funct Mol, Zhengzhou 450001, Henan, Peoples R China
[2] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA
[3] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 03期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
RESONANCE ENERGY-TRANSFER; MESSENGER-RNA; LIVING CELLS; DNA; AMPLIFICATION; GENES;
D O I：
10.1039/c9cc08598j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
By assembling DNAzyme on DNA nanowires through DNA hybridization, we have developed a novel accelerated DNAzyme-based fluorescent nanoprobe for fast, sensitive and selective detection of miRNA. Moreover, the strategy was successfully applied for in situ imaging of miRNA-21 in different cell lines.
引用
收藏
页码：470 / 473
页数：4
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