Isolation of Microglia from Mouse or Human Tissue

被引：54

作者：

Bordt, Evan A. ^{[1
]}

Block, Carina L. ^{[2
]}

Petrozziello, Tiziana ^{[3
]}

Sadri-Vakili, Ghazaleh ^{[3
]}

Smith, Caroline J. ^{[1
,2
]}

Edlow, Andrea G. ^{[4
]}

Bilbo, Staci D. ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Lurie Ctr Autism, Harvard Med Sch, Dept Pediat, Boston, MA 02114 USA

[2] Duke Univ, Dept Psychol & Neurosci, Durham, NC 27708 USA

[3] Massachusetts Gen Hosp, Healey Ctr ALS MassGeneral Hosp, NeuroEpigenet Lab, Boston, MA 02114 USA

[4] Massachusetts Gen Hosp, Vincent Ctr Reprod Biol, Harvard Med Sch, Obstet Gynecology & Reproductive Biol, Boston, MA 02114 USA

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 01期

关键词：

D O I：

10.1016/j.xpro.2020.100035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries.For complete details on the use and execution of this protocol, please refer to Hanamsagar et al. (2017), Rivera et al. (2019), and Edlow et al. (2019)

引用

页数：18

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