Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium

被引:78
|
作者
Johjima, T
Itoh, N
Kabuto, M
Tokimura, F
Nakagawa, T
Wariishi, H [1 ]
Tanaka, H
机构
[1] Kyushu Univ, Dept Forest Prod, Higashi Ku, Fukuoka 8128581, Japan
[2] Nissei Sangyo Co Ltd, Tokyo 1050004, Japan
关键词
D O I
10.1073/pnas.96.5.1989
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 mu M. The LiP-DHP interaction was controlled by the ionization group with a pK(a) of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 mu M, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s(-1), respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.
引用
收藏
页码:1989 / 1994
页数:6
相关论文
共 50 条
  • [1] Direct interaction of lignin and lignin peroxidase from basidiomycete Phanerochaete chrysosporium
    Johjima, T
    Itoh, N
    Wariishi, H
    Tanaka, H
    [J]. FASEB JOURNAL, 1997, 11 (09): : A1319 - A1319
  • [2] LIGNIN PEROXIDASE OF PHANEROCHAETE-CHRYSOSPORIUM
    TIEN, M
    KIRK, TK
    [J]. METHODS IN ENZYMOLOGY, 1988, 161 : 238 - 249
  • [3] LIGNIN PEROXIDASE FROM FUNGI - PHANEROCHAETE-CHRYSOSPORIUM
    KIRK, TK
    TIEN, M
    KERSTEN, PJ
    KALYANARAMAN, B
    HAMMEL, KE
    FARRELL, RL
    [J]. METHODS IN ENZYMOLOGY, 1990, 188 : 159 - 171
  • [4] Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
    Woo S.H.
    Cho J.S.
    Lee B.S.
    Kim E.K.
    [J]. Biotechnology and Bioprocess Engineering, 2004, 9 (4) : 256 - 260
  • [5] Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
    Woo, SH
    Cho, JS
    Lee, BS
    Kim, EK
    [J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2004, 9 (04) : 256 - 260
  • [6] Cultivation of Phanerochaete chrysosporium and lignin peroxidase activity
    Kim, YK
    Kim, G
    Jeong, MS
    [J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 6 (06) : 420 - 424
  • [7] PRODUCTION OF PHANEROCHAETE-CHRYSOSPORIUM LIGNIN PEROXIDASE
    LINKO, S
    [J]. BIOTECHNOLOGY ADVANCES, 1992, 10 (02) : 191 - 236
  • [8] Oxidation of aromatic sulfides by lignin peroxidase from Phanerochaete chrysosporium
    Baciocchi, E
    Gerini, MF
    Harvey, PJ
    Lanzalunga, O
    Mancinelli, S
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (09): : 2705 - 2710
  • [9] INDUCTION AND STABILIZATION OF LIGNIN PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM
    SANG, BI
    KIM, YH
    YOO, YJ
    [J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1995, 5 (04) : 218 - 223
  • [10] Enzymatic decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
    Beenish Sadaqat
    Nazia Khatoon
    Aneela Younas Malik
    Asif Jamal
    Uzma Farooq
    Muhammad Ishtiaq Ali
    Huan He
    Fang-Jing Liu
    Hongguang Guo
    Michael Urynowicz
    Qiurong Wang
    Zaixing Huang
    [J]. Scientific Reports, 10