RELATIONSHIP BETWEEN SINGLE NUCLEOTIDE POLYMORPHISM OF DNA REPAIR GENES ERCC1 AND ERCC2 AND CERVICAL CANCER SUSCEPTIBILITY

Objective: To analyze the relationship between the single nucleotide polymorphisms of DNA repair genes ERCC1 and ERCC2 and cervical cancer susceptibility. Methods: A total of 100 cervical cancer patients admitted to our hospital from March 2017 to March 2018 were enrolled in the observation group, and 100 healthy women who came to our hospital for physical examination during the same period were recruited to the control group. General data such as age, menarche age, number of pregnancies, number of deliveries, and smoking history were collected. DNA was extracted using the Qiagen Blood Kit blood genomic DNA kit produced by Qiagen, and the polymorphisms of ERCC1 rs11615 and rs3212986, and ERCC2 rs13181 and rs1799793 genes were detected using MALD1-T0F technology. The relationship between ERCC1 (rs11615 and rs3212986) and ERCC2 (rs13181 and rs1799793) gene polymorphisms and cervical cancer susceptibility was analyzed using conditional logistic regression analysis. Results: The age of menarche and age of first sexual life in the observation group were significantly lower than those in the control group, and the difference was statistically significant (P<0.05), but there was no statistically significant difference in other general data between the two groups (P>0.05). There was no significant difference in the frequency distribution of ERCC1 rs11615 and rs3212986, and ERCC2 rs13181 and rs1799793 genes between the two groups ( P>0.05). The findings of logistic regression analysis showed that the individuals with ERCC1 rs11615 carrying TT genotype [3.55 (0.84-16.59)] were at much higher risk of cervical cancer than those carrying CC and CT genotypes, while ERCC1 rs3212986 and ERCC2 rs13181 and rsI799793 gene polymorphisms had no obvious relationship with cervical cancer. Conclusions: The DNA repair gene ERCC1 rs11615 carrying TT genotype is likely to increase the risk of cervical cancer, but there was no correlation between the ERCC2 gene polymorphism and cervical cancer.