Rapid identification of Panax ginseng and Panax quinquefolius using SNP-based probes

被引：0
|
作者：
Wang, R. L. [1 ]
Gu, D. J. [1 ]
Hou, C. R. [1 ]
Liu, Q. J. [1 ]
机构：
[1] Southeast Univ, State Key Lab Bioelect, Nanjing, Jiangsu, Peoples R China
来源：
BIOINFORMATICS AND BIOMEDICAL ENGINEERING: NEW ADVANCES | 2016年
基金：
中国国家自然科学基金;
关键词：
AUTHENTICATION; DIVERSITY; ARRAYS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An effective, easy and rapid method of distinguishing Panax species is urgently needed, for traditional methods are time-consuming and inaccurate. Here, universal primer for multiplex PCR, and probes containing the 2 SNPs and an additional mismatch were designed in ITS2 region to distinguish P. ginseng and P. quinquefolius. In this system, multiplex amplification was integrated with hybridization, making the single test costing only 2-4 h. The detection sensitivity of this system reached 2 pg per reaction. Results showed the system can be applied to effectively identification of P. ginseng and P. quinquefolius.
引用
收藏
页码：319 / 324
页数：6
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