Colorimetric Immunoassay for Rapid Detection of Vibrio parahemolyticus Based on Mn2+ Mediates the Assembly of Gold Nanoparticles

被引：47

作者：

Fu, Kaiyue ^{[1
]}

Zheng, Yan ^{[1
,2
]}

Li, Juan ^{[1
]}

Liu, Yushen ^{[1
]}

Pang, Bo ^{[1
]}

Song, Xiuling ^{[1
]}

Xu, Kun ^{[1
]}

Wang, Juan ^{[1
]}

Zhao, Chao ^{[1
]}

机构：

[1] Jilin Univ, Sch Publ Hlth, Changchun 130021, Jilin, Peoples R China

[2] Jilin Univ, Dept Cadre Ward, Hosp 1, Changchun 130021, Jilin, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 36期

基金：

中国博士后科学基金;

关键词：

food safety; Vibrio parahemolyticus; colorimetric immunoassay; magnetic beads; MnO2; nanoparticles; gold nanoparticles sensing system; LISTERIA-MONOCYTOGENES; SENSITIVE DETECTION; PARAHAEMOLYTICUS; PEROXIDASE; BACTERIA; SEAFOOD; MIMICS; SENSOR; IONS; H2O2;

D O I：

10.1021/acs.jafc.8b02494

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Vibrio parahemolyticus (V. parahemolyticus) is an important food-borne pathogen that causes food poisoning and acute gastroenteritis in humans. Herein, a novel colorimetric immunoassay was presented for rapid detection of V. parahemolyticus using gold nanoparticles (18.1 nm diameter) as chromogenic substrate, whose combination of a magnetic bead based sandwich immunoassay and an optical sensing system via Mn2+ ions mediated aggregation of gold nanoparticles. MnO, nanoparticles coated with polyclonal IgG antibodies (7.8 nm diameter) are used to recognize the target and can be etched to generate manganese ions by ascorbic acid. A color change ranging from red to purple to blue can be easily discerned by bare eye, corresponding to V. parahemolyticus concentration in the range between 10 and 106 cfu.mL(-1). The proposed method possesses high specificity with a limit of detection of 10 cfu.mL(-1) and was successfully applied to determination of V. parahemolyticus in oyster samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of V. parahemolyticus.

引用

页码：9516 / 9521

页数：6