Simulation and Experiment for Electrode Coverage Evaluation by Electrochemical Impedance Spectroscopy Using Parallel Facing Electrodes

被引：3
|
作者：
Tanaka, Shinya [1 ]
Kimura, Kaiken [1 ]
Miyamoto, Ko-ichiro [2 ]
Yanase, Yuhki [3 ]
Uno, Shigeyasu [1 ]
机构：
[1] Ritsumeikan Univ, Grad Sch Sci & Engn, Dept Elect Syst, 1-1-1 Nojihigashi, Kusatsu, Shiga 5258577, Japan
[2] Tohoku Univ, Dept Elect Engn, Aoba Ku, 6-6-05 Aza Aoba, Sendai, Miyagi 9808579, Japan
[3] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Dermatol, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348551, Japan
来源：
ANALYTICAL SCIENCES | 2020年 / 36卷 / 07期
关键词：
Electrochemical impedance spectroscopy; electrochemical sensor; finite element method simulation; CELL; LABEL; DIFFERENTIATION; SENSITIVITY; CYTOMETER; ADHESION; FIELD;
D O I：
10.2116/analsci.19P451
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A parallel facing electrode (PFE) structure for adherent cell monitoring by electrochemical impedance spectroscopy (EIS) was developed, and its characteristics were investigated by both computer simulation and experiment. The PFE model consists of two facing gold electrode strips separated by 40 mu m, and the area of its intersection is 500 x 500 mu m. Computer simulation of EIS with adherent cells showed a distinct difference in solution resistance for different cell coverage, which was confirmed by experimental results using latex beads suspension. A well-defined relationship between solution resistance and cell coverage in our PFE is promising for quantitative evaluation of cell density, morphology and fatality.
引用
收藏
页码：853 / 858
页数：6
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