Efficient production of transgenic bovine by microinjection and cloning technology of early embryos

The present results suggested the feasibility of EGFP-gene for non-invasive selection of transgenic bovine embryos at the pre-implantation stage by using the fluorescence microscope and linking the marker gene contacted with a desired gene. However, these injected embryos showed impaired development and high rate of the mosaic expression. Therefore, we conducted to transfer the EGFP gene fragment into the bovine fetus fibroblasts using Liposome for expressing bright green fluorescence in the whole part ICM. EGFP has a great advantage as a marker, cells or organ can be observed at any time in their viable and intact and easily connected with pharmaceutical protein in vitro. Additional advantages of nuclear transfer combined with transgenic become obvious when it is compared with microinjection techniques. In the future, we can produce the pharmaceutical proteins from cow using nuclear transfer with EGFP gene.