The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing

被引:17
|
作者
Anbu, Sellamuthu [1 ]
Kamalraj, Subban [2 ]
Paul, Anup [1 ]
Jayabaskaran, Chelliah [2 ]
Pombeiro, Armando J. L. [1 ]
机构
[1] Univ Lisbon, Ctr Quim Estrutural, Inst Super Tecn, Complexo 1,Av Rovisco Pais, P-1049001 Lisbon, Portugal
[2] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India
关键词
SELECTIVE RECOGNITION; PHOSPHATE; CHEMOSENSOR; DESIGN; ATP; SENSORS; ANIONS; COORDINATION; RECEPTORS; BINDING;
D O I
10.1039/c4dt03590a
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
A highly selective and sensitive phenanthroimidazole tagged Mannich base type dizinc(II) fluorescent probe (R-Zn2+) has been developed for the pyrophosphate ion (PPi) with a very low limit of detection (LOD) of 0.25 ppm; this also assesses PPi from DNA polymerization chain reaction (PCR).
引用
收藏
页码:3930 / 3933
页数:4
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