A 3D Spheroid Model for Glioblastoma

被引：18
|
作者：
Guyon, Joris [1 ,2 ]
Andrique, Laetitia [1 ,2 ]
Pujol, Nadege [1 ,2 ]
Rosland, Gro Vatne [3 ,4 ]
Recher, Gaelle [1 ,5 ,6 ]
Bikfalvi, Andreas [1 ,2 ]
Daubon, Thomas [1 ,2 ,4 ]
机构：
[1] Univ Bordeaux, LAMC, Bordeaux, France
[2] Univ Bordeaux, INSERM U1029, Bordeaux, France
[3] Univ Bergen, Dept Biomed, Bergen, Norway
[4] CNRS, IBGC UMR5095, Paris, France
[5] CNRS, LP2N, IOA, UMR 5298, Paris, France
[6] IOA, Inst Opt Grad Sch, Talence, France
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2020年 / 158期
关键词：
Cancer Research; Issue; 158; glioblastoma; patient-derived cell; spheroid; invasion; migration; proliferation; in vitro models; MIGRATION; CELL;
D O I：
10.3791/60998
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Two-dimensional (2D) cell cultures do not mimic in vivo tumor growth satisfactorily. Therefore, three-dimensional (3D) culture spheroid models were developed. These models may be particularly important in the field of neuro-oncology. Indeed, brain tumors have the tendency to invade the healthy brain environment. We describe herein an ideal 3D glioblastoma spheroid-based assay that we developed to study tumor invasion. We provide all technical details and analytical tools to successfully perform this assay.
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收藏
页数：8
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