Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR

被引：20
|
作者：
Cremazy, F [1 ]
Soullier, S [1 ]
Berta, P [1 ]
Jay, P [1 ]
机构：
[1] Inst Genet Humaine, UPR CNRS 1142, Human Mol Genet Grp, F-34396 Montpellier 5, France
来源：
FEBS LETTERS | 1998年 / 438卷 / 03期
基金：
澳大利亚研究理事会;
关键词：
SOX; SRY-related; HMG; nested polymerase chain reaction; multigene family; human;
D O I：
10.1016/S0014-5793(98)01294-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SOX proteins contain a conserved HMG-related DNA-binding domain. They fulfil essential functions during the development of animals. Mutations in several SOX genes have been implicated in human diseases, We present here a new set of PCR primers designed to amplify a broad range of SOX HMG-box sequences. These primers facilitated the cloning of several new SOX HMG boxes from human genomic DNA, revealing unexpected complexity of the SOX gene family. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：311 / 314
页数：4
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