Micropropagation of Populus euphratica Olivier

Micropropagation of the stress tolerant tree Populus euphratica was achieved via induction of adventitious shoots on calli of leaf explants. Callus was induced on half strength MS medium containing 1.0 muM BAP and 2.5 muM NAA. In darkness calli produced large numbers of adventitious shoots. Shoots were multiplied on media containing 2.5 mm BAP and elongated on WPM supplemented with 2.5 mM BAP and 0.05 mu NAA. Efficient and fast rooting of the elongated shoots was obtained in medium with a NH4+ : NO3 ratio of 1:4 with a total nitrogen concentration of 20 mM supplemented with 0.5 muM IBA. This report provides a protocol for propagation of P. euphratica including transfer to the greenhouse.