Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination

被引：3

作者：

Fresco-Taboada, Alba ^{[1
]}

Garcia-Duran, Marga ^{[1
]}

Aira, Cristina ^{[1
]}

Lopez, Lissett ^{[1
]}

Sastre, Patricia ^{[1
]}

van der Hoek, Lia ^{[2
]}

van Gils, Marit J. ^{[2
]}

Brouwer, Philip J. M. ^{[2
]}

Sanders, Rogier W. ^{[2
]}

Holzer, Barbara ^{[3
]}

Zimpernikc, Irene ^{[3
]}

Lopez-Collazo, Eduardo ^{[4
,5
,6
,7
]}

Munoz, Patricia ^{[8
]}

Rueda, Paloma ^{[1
]}

Vela, Carmen ^{[1
]}

机构：

[1] Eurofins Ingenasa, Madrid, Spain

[2] Amsterdam UMC, Amsterdam Inst Infect & Immun, Dept Med Microbiol & Infect Prevent, Lab Expt Virol, Amsterdam, Netherlands

[3] Austrian Agcy Hlth & Food Safety, Dept Serol & Virol, Modling, Austria

[4] La Paz Univ Hosp, Innate Immune Response Grp, IdiPAZ, Madrid, Spain

[5] La Paz Univ Hosp, Tummor Immunol Lab, IdiPAZ, Madrid, Spain

[6] La Paz Univ Hosp, Emergency Dept, Madrid, Spain

[7] La Paz Univ Hosp, Emergent Pathol Res Grp, IdiPAZ, Madrid, Spain

[8] Univ Complutense Madrid, Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis, Madrid, Spain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2022年 / 102卷 / 04期

关键词：

SARS-CoV-2; COVID-19; Vaccination; Serology; RBD; Spike protein;

D O I：

10.1016/j.diagmicrobio.2022.115650

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Massive vaccination programs are being carried out to limit the SARS-CoV-2 pandemic that started in December 2019. Serological tests are of major importance as an indicator of circulation of the virus and to assess how vaccine-induced immunity progresses. An Enzyme-Linked Immunosorbent Assay (ELISA) and a Lateral Flow Assay (LFA) have been developed based on the SARS-CoV-2 recombinant Receptor Binding Domain (RBD) and the combination of Spike and Nucleoprotein, respectively. The validation with 1272 serum samples by comparison with INgezim COVID 19 DR showed good diagnostic performance (sensitivity: 93.2%-97.2%; specificity: 98.3%-99.3%) for detection of previous contact with SARS-CoV-2. Moreover, according to our results, these assays can help in the serosurveillance during and after vaccination, by detecting the humoral immune response as soon as 15 days post vaccination and identifying low-respondents. Hence, these tests could play a key role in the progression to a COVID-19 free world, helping to adjust future vaccination protocols. (C) 2022 Elsevier Inc. All rights reserved.

引用

页数：7

共 50 条

[1] Diagnostic Performance of Serological Assays in the Detection of SARS-CoV-2: A Review
Carinci, Francesco
Moreo, Giulia
Limongelli, Luisa
Testori, Tiziano
Lauritano, Dorina
[J]. APPLIED SCIENCES-BASEL, 2020, 10 (13):
[2] Comparison of Serological Assays for the Detection of SARS-CoV-2 Antibodies
James, Joe
Rhodes, Shelley
Ross, Craig S.
Skinner, Paul
Smith, Samuel P.
Shipley, Rebecca
Warren, Caroline J.
Goharriz, Hooman
McElhinney, Lorraine M.
Temperton, Nigel
Wright, Edward
Fooks, Anthony R.
Clark, Tristan W.
Brookes, Sharon M.
Brown, Ian H.
Banyard, Ashley C.
[J]. VIRUSES-BASEL, 2021, 13 (04):
[3] Evaluation of diagnostic accuracy of 10 serological assays for detection of SARS-CoV-2 antibodies
Ainhoa Gutiérrez-Cobos
Sara Gómez de Frutos
Diego Domingo García
Eva Navarro Lara
Ayla Yarci Carrión
Leticia Fontán García-Rodrigo
Arturo Manuel Fraile Torres
Laura Cardeñoso Domingo
[J]. European Journal of Clinical Microbiology & Infectious Diseases, 2021, 40 : 955 - 961
[4] Evaluation of diagnostic accuracy of 10 serological assays for detection of SARS-CoV-2 antibodies
Gutierrez-Cobos, Ainhoa
Gomez de Frutos, Sara
Domingo Garcia, Diego
Navarro Lara, Eva
Yarci Carrion, Ayla
Fontan Garcia-Rodrigo, Leticia
Fraile Torres, Arturo Manuel
Cardenoso Domingo, Laura
[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2021, 40 (05) : 955 - 961
[5] Surveillance of Immunological Status after Vaccination by two Serological Assays based on SARS-CoV-2 Spike Protein
Fresco-Taboada, A.
Garcia-Duran, M.
Aira, C.
Lopez, L.
Sastre, P.
Van der Hoek, L.
Van Gils, M.
Brouwer, P. J. M.
Sanders, R. W.
Holzer, B.
Zimpernik, I.
Lopez-Collazo, E.
Munoz, P.
Rueda, P.
Vela, C.
[J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2022, 116 : S40 - S41
[6] Performance of Commercially Available Rapid Serological Assays for the Detection of SARS-CoV-2 Antibodies
Hashem, Anwar M.
Alhabbab, Rowa Y.
Algaissi, Abdullah
Alfaleh, Mohamed A.
Hala, Sharif
Abujamel, Turki S.
Elassouli, M-Zaki
AL-Somali, Afrah A.
Alofi, Fadwa S.
Khogeer, Asim A.
Alkayyal, Almohanad A.
Mahmoud, Ahmad Bakur
Almontashiri, Naif A. M.
Pain, Arnab
[J]. PATHOGENS, 2020, 9 (12): : 1 - 10
[7] Comparison of Five Serological Assays for the Detection of SARS-CoV-2 Antibodies
Doerschug, Anja
Schwanbeck, Julian
Hahn, Andreas
Hillebrecht, Anke
Blaschke, Sabine
Mese, Kemal
Gross, Uwe
Dierks, Sascha
Frickmann, Hagen
Zautner, Andreas E.
[J]. DIAGNOSTICS, 2021, 11 (01)
[8] Monitoring of SARS-CoV-2 Specific Antibodies after Vaccination
Guiomar, Raquel
Santos, Ana Joao
Melo, Aryse Martins
Costa, Ines
Matos, Rita
Rodrigues, Ana Paula
Kislaya, Irina
Silva, Anabela Santos
Roque, Carla
Nunes, Carla
Aguiar, Joaquim
Graca, Fatima
Silva Graca, Antonio
Machado, Ausenda
[J]. VACCINES, 2022, 10 (02)
[9] Serological Tests in the Detection of SARS-CoV-2 Antibodies
Guevara-Hoyer, Kissy
Fuentes-Antras, Jesus
De la Fuente-Munoz, Eduardo
Rodriguez de la Pena, Antonia
Vinuela, Marcos
Cabello-Clotet, Noemi
Estrada, Vicente
Culebras, Esther
Delgado-Iribarren, Alberto
Martinez-Novillo, Mercedes
Jose Torrejon, Maria
Perez de Diego, Rebeca
Fernandez-Arquero, Miguel
Ocana, Alberto
Perez-Segura, Pedro
Sanchez-Ramon, Silvia
[J]. DIAGNOSTICS, 2021, 11 (04)
[10] Rapid Serological Tests for SARS-CoV-2: Diagnostic Performance of 4 Commercial Assays
de Almeida, Sergio M.
Spalanzani, Regiane N.
Nogueira, Meri B.
Sanada, Beatriz
Cavalli, Barbara M.
Rotta, Indianara
Takahashi, Gislene R. A.
Pereira, Luciane A.
Moreira, Francielli B.
Dino, Carolina L. T.
Graf, Maria E.
de Almeida, Bernardo M. M.
Domino, Natalia R.
Genelhoud, Gustavo
Bochnia-Bueno, Lucas
Raboni, Sonia M.
[J]. MEDICAL PRINCIPLES AND PRACTICE, 2021, 30 (04) : 385 - 394

← 1 2 3 4 5 →