2,3,5-triphenyltetrazolium chloride (TTC) as electron acceptor of culturable soil bacteria, fungi and actinomycetes

被引：58
|
作者：
Praveen-Kumar [1 ]
Tarafdar, JC [1 ]
机构：
[1] Cent Arid Zone Res Inst, Jodhpur 342003, Rajasthan, India
来源：
BIOLOGY AND FERTILITY OF SOILS | 2003年 / 38卷 / 03期
关键词：
actinomycetes; bacteria; dehydrogenase activity; electron acceptor; fungi; TTC;
D O I：
10.1007/s00374-003-0600-y
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Dehydrogenase assays based on the reduction of 2,3,5- triphenyltetrazolium chloride (TTC) to the creaming red-coloured formazan (TPF), have been used to determine microbial activity in soil. By spraying TTC on culturable microbial colonies, whose growth was induced by applying glucose, we observed that from 72 to 100% of the bacterial and actinomycetes colonies in different soils were capable of using TTC as an electron acceptor. This percentage decreased to only 5% in the case of fungal colonies.
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页码：186 / 189
页数：4
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