Rapid Impedance Measurement of Tethered Bilayer Lipid Membrane Biosensors

被引：0
|
作者：
Mu, Xiaoyi [1 ]
Rairigh, Daniel [1 ]
Liu, Xiaowen [1 ]
Mason, Andrew J. [1 ]
机构：
[1] Michigan State Univ, E Lansing, MI 48824 USA
来源：
2011 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2011年
关键词：
SPECTROSCOPY;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Tethered bilayer lipid membranes (tBLM) offer a promising means to immobilize membrane proteins for sensor applications and study biological phenomena including membrane-nanoparticle interactions. tBLM biointerfaces are typically characterized using electrochemical impedance spectroscopy (EIS) in the 1mHz to 1Hz range due to interface parasitics. To enable rapid characterization of biointerfaces for high throughput applications, this paper introduces a method for high resolution EIS characterization of tBLMs at higher frequencies. The tBLM equivalent electrical model is analyzed, and the benefit of extracting the real portion of interface admittance is described. Mathematical analysis shows that the maximum frequency for measuring membrane resistance is a function of membrane characteristics and that small area membranes could enable measurement well into the kHz range, permitting observation of millisecond membrane protein activity in biosensor arrays.
引用
收藏
页码：4796 / 4799
页数：4
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