Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration

被引：5
|
作者：
Guilbaud, Mickael [1 ,2 ,3 ]
Chadeuf, Gilliane [1 ,2 ]
Avolio, Fabio [4 ]
Francois, Achille [1 ,2 ]
Moullier, Philippe [1 ,2 ,3 ]
Recchia, Alessandra [4 ,5 ]
Salvetti, Anna [1 ,2 ]
机构：
[1] Ecole Normale Super Lyon, INSERM, U758, F-69007 Lyon, France
[2] INSERM, U649, F-44000 Nantes, France
[3] EFS Pays Loire, F-4400 Nantes, France
[4] Ist Sci San Raffaele, Gene Therapy Program, Milan, Italy
[5] Univ Modena, Dept Biomed Sci, I-41100 Modena, Italy
来源：
JOURNAL OF VIROLOGY | 2008年 / 82卷 / 05期
关键词：
D O I：
10.1128/JVI.01956-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
引用
收藏
页码：2590 / 2593
页数：4
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