Electrochemical Behaviors of Horseradish Peroxidase on MoS2 Nanosheets Modified Electrode

In this paper molybdenum disulfide (MoS2) nanosheets and horseradish peroxidase (HRP) modified electrode was prepared with ionic liquid 1-hexylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE). UV-Vis and FT-IR spectra showed that the native framework of HRP was sustained after mixed with MoS2 nanosheet. As a 2D layered nanosheet MoS2 acted as the electron transfer bridge for HRP. A pair of well-defined redox peaks of HRP appeared on MoS2/CILE, implying the realization of direct electron transfer. Electrochemical parameters of HRP were calculated according to the cyclic voltammetric data with the electron transfer coefficient and electron transfer rate constant as 0.51 and 1.65 s(-1). The HRP modified electrode exhibited a prominent electrocatalytic behavior towards the reduction of trichloroacetic acid with wider dynamic range and lower detection limit of 0.67 mmol L-1 (3 sigma).