Baicalin improves podocyte injury in rats with diabetic nephropathy by inhibiting PI3K/Akt/ mTOR signaling pathway

Objective - To investigate the effect of baicalin on diabetic nephropathy (DN) rats and podocytes and its mechanism. Methods - The rat models with DN were established by high-fat and high-sugar diet and intraperitoneal injection of streptozotocin. The fasting blood glucose (FBG) and weight of rats in each group were measured at 0, 1, 2, 3, and 4 weeks. Their biochemical indicators, expression of inflammatory, and antioxidant factors were measured using an automatic biochemical analyzer together with ELISA. Hematoxylin-eosin staining and periodic acid-schiff staining were used to observe the morphological changes in the kidneys of rats in each group. Finally, the expressions of related molecules and PI3K/Akt/mTOR signaling pathway proteins in renal tissues and podocytes were examined by qRT-PCR and Western blot. Results - Compared with the DN group, the FBG and weight, serum creatinine, blood urea nitrogen, total cho-lesterol, triacylglycerol, microalbumin, and albumin/crea-tinine ratio were all significantly decreased in the Baicalin treatment groups in a concentration-dependent manner. The levels of inflammatory factors in kidney tissue and podocytes were decreased. In addition, the activities of lactate dehydrogenase and malondialdehyde in tissue were decreased, while the superoxide dismutase was increased. The pathological sections showed that glomerular atrophy and glomerular basement membrane thickening caused by hyperglycemia were improved in the Baicalin treatment groups. Meanwhile, baicalin inhibited the downregulation of Nephrin and Podocin expressions and upregulation of Desmin expression caused by DN, and inhibited the expressions of p-PI3K, p-Akt, and p-mTOR proteins. Conclusion - Baicalin slows down podocyte injury caused by DN by inhibiting the activity of PI3K/Akt/ mTOR signaling pathway.