Mucilaginibacter roseus sp nov., isolated from a freshwater river

A bacterial strain, designated TTM-1(T), was isolated from a water sample taken from the Caohu River in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain TTM-1(T) were Gram-stain-negative, aerobic, non-motile, rod-shaped and covered by large capsules, and formed pink-coloured colonies. Growth occurred at 10-37 degrees C (optimum 30-37 degrees C), at pH 6-8 (optimum pH 6-7) and with 0-2 % NaCl (optimum 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-1(T) belonged to the genus Mucilaginibacter and was most closely related to Mucilaginibacter defluvii A5(T) with a 16S rRNA gene sequence similarity of 97.3 %. The predominant fatty acids of strain TTM-1(T) were summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c; 37.1 %) and iso-C-15 : 0 (30.7 %). The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was MK-7. The DNA G + C content of the genomic DNA was 45.1 mol%. The DNA-DNA relatedness of strain TTM-1(T) with respect to recognized species of the genus Mucilaginibacter was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-1(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter roseus sp. nov. is proposed. The type strain is TTM-1(T) (=LMG 28454(T) =KCTC 42273(T)).