Comparison of diagnostic performance of five molecular assays for detection of SARS-CoV-2

被引：7
|
作者：
Kanwar, Neena [1 ,2 ]
Banerjee, Dithi [1 ,2 ]
Sasidharan, Anjana [1 ]
Abdulhamid, Ayah [1 ]
Larson, Marissa [3 ]
Lee, Brian [2 ,4 ]
Selvarangan, Rangaraj [1 ,2 ,3 ]
Liesman, Rachael M. [3 ]
机构：
[1] Childrens Mercy, Dept Pathol & Lab Med, Kansas City, MO 64108 USA
[2] Univ Missouri, Sch Med, Kansas City, MO 64108 USA
[3] Univ Kansas Hlth Syst, Dept Pathol & Lab Med, Kansas City, KS 66160 USA
[4] Childrens Mercy, Div Hlth Serv & Outcomes Res, Kansas City, MO USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2021年 / 101卷 / 04期
关键词：
SARS-CoV-2; detection; Molecular assay comparison; COVID-19;
D O I：
10.1016/j.diagmicrobio.2021.115518
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We compared the performance of the Abbott Real Time SARS-CoV-2 assay (Abbott assay), AptimaTM SARSCoV-2 assay (Aptima assay), BGI Real-Time SARS-CoV-2 assay (BGI assay), Lyra (R) SARS-CoV-2 assay (Lyra assay), and DiaSorin SimplexaTM COVID assay for SARS-CoV-2 detection. Residual nasopharyngeal samples (n = 201) submitted for routine SARS-CoV-2 testing by Simplexa assay during June-July 2020 and January 2021 were salvaged. Aliquots were tested on other assays and compared against the CDC 201 9-nCoV Real-Time RT-PCR assay. Viral load in positive samples was determined by droplet digital PCR. Among 201 samples, 99 were positive and 102 were negative by the CDC assay. The Aptima and Abbott assays exhibited the highest positive percent agreement (PPA) at 98.9% while the BGI assay demonstrated the lowest PPA of 89.9% with 10 missed detections. Negative percent agreement for all 5 platforms was comparable, ranging from 96.1% to 100%. The performance of all five assays was comparable. (C) 2021 Elsevier Inc. All rights reserved.
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页数：7
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