Marine Bacterium Derived Lipopeptides: Characterization and Cytotoxic Activity Against Cancer Cell Lines

被引：53
|
作者：
Sivapathasekaran, C. [1 ]
Das, Palashpriya [1 ]
Mukherjee, Soumen [1 ]
Saravanakumar, J. [2 ]
Mandal, Mahitosh [2 ]
Sen, Ramkrishna [1 ]
机构：
[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
[2] Indian Inst Technol, Sch Med Sci & Technol, Kharagpur 721302, W Bengal, India
来源：
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2010年 / 16卷 / 04期
关键词：
Marine bacterium; HPTLC; Lipopeptides; FTIR; MALDI-ToF; Antiproliferative activity; MASS-SPECTROMETRY; BACILLUS; BIOSURFACTANTS; APOPTOSIS; INDUCTION; RECOVERY;
D O I：
10.1007/s10989-010-9212-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A marine Bacillus circulans DMS-2 was able to grow and produce biosurfactant on glucose mineral salts medium (GMSM) with a reduction in the surface tension up to 27 mN m(-1). The microorganism produced 1.64 +/- A 0.1 g l(-1) of crude biosurfactant. The lipopeptide nature of the produced biosurfactant was confirmed by primulin and ninhydrin assays using High Performance Thin Layer Chromatography (HPTLC). Preparative thin layer chromatography (TLC) was performed to purify the lipopeptides from the crude biosurfactant. The critical micelle concentrations (CMC) of the crude and purified products were found to be 90 and 40 mg l(-1) respectively. Fourier transform infrared spectrophotometer (FTIR) and matrix assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectral analysis revealed the identity of the produced lipopeptides as surfactin (m/z 1,023 Da) and fengycin (m/z 1,495 Da) isoforms. The purified marine lipopeptides displayed a significant antiproliferative activity against the human colon cancer cell lines HCT-15 (IC50 80 mu g ml(-1)) and HT-29 (IC50 120 mu g ml(-1)).
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页码：215 / 222
页数：8
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